Taken from Addgene’s guidelines [Link]
Antibiotic Concentrations
The following list shows recommended antibiotic concentrations for LB media or agar plates.AntibioticConcentrationAmpicillin100 μg/mLBleocin5 μg/mLCarbenicillin100 μg/mLChloramphenicol25 μg/mLCoumermycin25 μg/mLGentamycin10 μg/mLKanamycin50 μg/mLSpectinomycin50 μg/mLTetracycline10 μg/mL
Example: To make 100 mL of LB/ampicillin growth media, add 100 μL of a 100 mg/mL ampicillin stock (1000X stock) to 100 mL of LB.
Reagent List
Unless otherwise indicated, the antibiotic powder can be dissolved in H20. Addgene recommends making 1000X stock solutions and storing aliquots at -20oC.ReagentCatalog NumberAmpicillinEMD Chemicals #171254BleocinEMD Chemicals #203408CarbenicillinEMD Chemicals #205805ChloramphenicolEMD Chemicals #220551 (dissolve with EtOH)CoumermycinSigma #C9270 (dissolve with DMSO)GentamycinEMD Chemicals #345814KanamycinEMD Chemicals # 420311SpectinomycinEMD Chemicals # 567570TetracyclineEMD Chemicals # 583411
Please note that the catalog numbers given in the list above are only examples, and there are many additional companies that supply these reagents.
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