Helpful information and How to Guides
Microsoft Word is an entity on to its own and does crazy stuff...no more so when you try and insert an image into a document, only for Word to compress it to a blurry mess and then proceed to randomly move it upon every keystroke. There are no-sure fire solutions to this, but below is way to help improve things.…
With the help of my designer wife we have made a pattern and how to video for making larger masks that are great for men and those with a bigger face. They are double layered with a pocket for a filter, with great chin coverage and a super comfy pliable fit around the nose. Hope this is helpful for everyone as we all try and get thru Covid-19.
In the following powerpoint tutorial I run through how you can use ImageJ/FIJI to import your raw microscopy image files and then analyse and track individual cells to generate single cell fate maps. Note, unless you are also planning on quantifying fluorescent intensity data in your movies, I strongly suggest converting all of your image files to high quality JPEGs…
Here is a step-by-step guide that I made to help people export microscope images from ImageJ/FIJI and then import and alter colours/levels etc in Photoshop. The guide also shows you how to easily move from Photoshop to illustrator to make montage images etc for publication. PDF Download: Guide to FIJI-Photoshop Image manipulation
I often get asked how to uses Thresholds to measure things in Image J. There are some great guides on the web explaining how to use Thresholds in Image J, and here are a few that are well worth checking out [Link1][Link2]. Below are some of the Basic Steps for using Thresholds: Open your image and duplicate it (Image>Duplicate) On the…
Image J can be downloaded for free from here . This guide can also be downloaded as a complete PDF here: Measuring Cell Fluorescence using ImageJ Here is a very simple guide for determining the level of fluorescence in a given region (e.g nucleus) Select the cell of interest using any of the drawing/selection tools (i.e. rectangle, circle, polygon or freeform) From the Analyze menu…
Here is a recent talk I gave to some members of the public at the Garvan Institute of Medical Research. It is a very general and simple over-view of explaining 1) how cells in your body proliferate, 2) how this goes wrong in cancer, 3) the challenges we are facing in treating and killing cancer, and 4) most importantly how we…
Are you interested in Cancer Research and want to do a PhD at the Garvan? Then come along to the open day and where you can meet with all the supervisors, discover some amazing projects, and get all the information you need to start your career in Cancer Research. Information Date: Wednesday 10th of September 2014 Time: 1.45-5pm Venue: The Kinghorn Cancer Centre, Garvan…
UPDATE: ImpactStory is not free, as I first thought, they currently have a 30 day free trail, then the cost is $45/year. Its clear that judging a researchers output purely on the impact/quality of the journal they do/don't publish is not always the best way to accurately judge individual achievement and output. To that end, article level metrics have recently…
The Mitchison Lab has an excellent guide on staining and fixing cells for Actin and Microtubules which is worth reading [Link] Coverslips Most coverslips come with a fine film coating to stop them sticking to each other. This can reduce the ability of coating agents such as poly-L lysine from working properly, and can thus reduce the ability of cells to…