Category: Information and Guides
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Using Apple AirPod Pros 2 at Concerts
Concert Mode, aka Adaptive Transparency Turning on Adaptive Transparency will allow you to hear everything around you while capping loud noises to 85 dB. This means you can use your Apple AirPod Pro 2 as awesome earplugs at loud concerts. Just put your AirPods in your ears. Turn on transparency…
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Adding Figures/Images to Word Documents
Microsoft Word is an entity on to its own and does crazy stuff…no more so when you try and insert an image into a document, only for Word to compress it to a blurry mess and then proceed to randomly move it upon every keystroke. There are no-sure fire solutions…
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Big Boy Mask Pattern: How to guide
With the help of my designer wife we have made a pattern and how to video for making larger masks that are great for men and those with a bigger face. They are double layered with a pocket for a filter, with great chin coverage and a super comfy pliable…
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How to analyse single cells time-lapse movies using Fiji and/or Photoshop
In the following powerpoint tutorial I run through how you can use ImageJ/FIJI to import your raw microscopy image files and then analyse and track individual cells to generate single cell fate maps. Note, unless you are also planning on quantifying fluorescent intensity data in your movies, I strongly suggest…
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User guide: Export microscope images from ImageJ/FIJI into Adobe Photoshop and Illustrator
Here is a step-by-step guide that I made to help people export microscope images from ImageJ/FIJI and then import and alter colours/levels etc in Photoshop. The guide also shows you how to easily move from Photoshop to illustrator to make montage images etc for publication. PDF Download: Guide to FIJI-Photoshop Image…
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Using Thresholds to Measure and Quantify Cells in Image J
I often get asked how to uses Thresholds to measure things in Image J. There are some great guides on the web explaining how to use Thresholds in Image J, and here are a few that are well worth checking out [Link1][Link2]. Below are some of the Basic Steps for using…
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Using ImageJ to Measure Cell Fluorescence
Image J can be downloaded for free from here . This guide can also be downloaded as a complete PDF here: Measuring Cell Fluorescence using ImageJ Here is a very simple guide for determining the level of fluorescence in a given region (e.g nucleus) Select the cell of interest using any of the drawing/selection tools…
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Public Talk “Killing Cancer One Cell at a Time ” now on YouTube
Here is a recent talk I gave to some members of the public at the Garvan Institute of Medical Research. It is a very general and simple over-view of explaining 1) how cells in your body proliferate, 2) how this goes wrong in cancer, 3) the challenges we are facing in…
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2014 Garvan Cancer Division PhD Open Day, 10th September !
Are you interested in Cancer Research and want to do a PhD at the Garvan? Then come along to the open day and where you can meet with all the supervisors, discover some amazing projects, and get all the information you need to start your career in Cancer Research. Information Date: Wednesday…
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ImpactStory: A new way to track individual article metrics
UPDATE: ImpactStory is not free, as I first thought, they currently have a 30 day free trail, then the cost is $45/year. Its clear that judging a researchers output purely on the impact/quality of the journal they do/don’t publish is not always the best way to accurately judge individual achievement…
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Immunofluorescence Guide
The Mitchison Lab has an excellent guide on staining and fixing cells for Actin and Microtubules which is worth reading [Link] Coverslips Most coverslips come with a fine film coating to stop them sticking to each other. This can reduce the ability of coating agents such as poly-L lysine from working…
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How to Synchronise Mammalian Cells in Culture
General Guidelines These guides are written primarily for HeLa cells, but it should be possible to extend these to other cell lines with a bit of optimisation. 1) Make sure your cells are happy ! 2) Aim for around 75-85% confluence at the time of release. 3) Try and keep…
Andrew Burgess PhD 