andrewburgess
How to analyse single cells time-lapse movies using Fiji and/or Photoshop
In the following powerpoint tutorial I run through how you can use ImageJ/FIJI to import your raw microscopy image files and then analyse...
top of page
Blog
andrewburgess
Mar 16, 20181 min read
User guide: Export microscope images from ImageJ/FIJI into Adobe Photoshop and Illustrator
Here is a step-by-step guide that I made to help people export microscope images from ImageJ/FIJI and then import and alter...
2
andrewburgess
Aug 14, 20152 min read
Using Thresholds to Measure and Quantify Cells in Image J
I often get asked how to uses Thresholds to measure things in Image J. There are some great guides on the web explaining how to use...
0
andrewburgess
Aug 13, 20152 min read
Using ImageJ to Measure Cell Fluorescence
Image J can be downloaded for free from here . This guide can also be downloaded as a complete PDF here: Measuring Cell Fluorescence...
0
andrewburgess
Aug 13, 20144 min read
Immunofluorescence Guide
The Mitchison Lab has an excellent guide on staining and fixing cells for Actin and Microtubules which is worth reading [Link] Coverslips...
1
andrewburgess
Aug 13, 20145 min read
How to Synchronise Mammalian Cells in Culture
General Guidelines These guides are written primarily for HeLa cells, but it should be possible to extend these to other cell lines with...
4
andrewburgess
Aug 13, 20141 min read
HeLa SILAC conditions
Grow cells in DMEM (Sigma #D9443) without L-Arg, L-Leu, L-Lys ! This DMEM is low glucose 1000mg/L so you will need to add and extra...
0
andrewburgess
Aug 13, 20141 min read
Complete Guide to Cell Cycle based Flow Cytometry
A complete ‘ish’ guide to Flow cytometry or FACS for cell cycle work. Covers all aspects from tissue culture, through to running and...
0
andrewburgess
Aug 13, 20141 min read
Antibiotic Concentrations for Bacterial Work
Taken from Addgene’s guidelines [Link] Antibiotic Concentrations The following list shows recommended antibiotic concentrations for LB...
0
andrewburgess
Aug 13, 20141 min read
Adding a Scale Bar to Microscope Images
With the later versions of Photoshop CS4 and CS5 extended it is now very easy to add a scale bar to your microscope images. But before we...
0
andrewburgess
Mar 20, 20144 min read
IncuCyte Zoom Video Tutorial and User Guide
Here is a guide that was written by Rachael McCloy from our lab on how to use the IncuCyte Zoom Kinetic Imaging System. We recently...
1
andrewburgess
Aug 13, 20131 min read
Useful Links
Cancer Gene Database Searcher http://www.oncomine.org/ Protein Phosphorylation and other PTMs Database http://www.phosphosite.org/ Search...
0
andrewburgess
Aug 13, 20131 min read
SDS PAGE Gel Recipes
#Acrylamide #proteingels #SDSPAGE #westernblot
0
bottom of page